V5 tag antibody [SV5-Pk1] (ab27671)

Product Name 

V5 tag antibody [SV5-Pk1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [SV5-Pk1] to V5 tag

Immunogen 

Mice were infected with the paramyxovirus SV5, Simian-Virus 5.

Epitope 

Ab27671 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.

Tested applications 
(see key)

ELISA, ICC, IHC-Fr, IP, RIA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000 - 1/5000.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
WB: 1/1000 - 1/5000.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Tags & Cell Markers >> Epitope Tags >> V5 Tag

Relevance 

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

SV5-Pk1

Myeloma 

Sp2/0-Ag14

Isotype 

IgG2a

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody was purified from tissue culture supernatant by affinity chromatography.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

References for V5 tag antibody [SV5-Pk1] (ab27671)

  This product has been used in: (two most recent references)

Gopalakrishnan J et al. Self-assembling SAS-6 multimer is a core centriole building block. J Biol Chem 285:8759-70 (2010). WB. PubMed: 20083610

This clone has been used in:
If you publish research using ab27671 please let us know so that we can cite the reference on this datasheet.

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