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Anti-V5 tag antibody [SV5-Pk1] (ab27671)

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Overview

Product name

Anti-V5 tag antibody [SV5-Pk1]
V5 tag 抗体 (24件) 一覧

Description

Mouse monoclonal [SV5-Pk1] to V5 tag

Tested applications

ICC/IF, ICC, IHC-Fr, ELISA, IP, WB, RIAmore details

Immunogen

Mice were infected with the paramyxovirus SV5, Simian-Virus 5.

Epitope

Ab27671 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

This antibody was purified from tissue culture supernatant by affinity chromatography.

Primary antibody notes

Ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.

Clonality

Monoclonal

Clone number

SV5-Pk1

Myeloma

Sp2/0-Ag14

Isotype

IgG2a

Research areas

Tags & Cell Markers >> Epitope Tags >> V5 Tag

  • Western blot - V5 tag antibody [SV5-Pk1] (ab27671)Western blot - V5 tag antibody [SV5-Pk1] (ab27671) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - V5 tag antibody [SV5-Pk1] (ab27671)Immunocytochemistry/ Immunofluorescence - V5 tag antibody [SV5-Pk1] (ab27671) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab27671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Cellular localization

Not applicable

Alternative names

  • GKPIPNPLLGLDST epitope tag antibody
  • GKPIPNPLLGLDST tag antibody
  • Protein Rev antibody
  • Regulator of expression of viral proteins antibody
  • rev antibody
  • V5 epitope tag antibody
see all

Anti-V5 tag antibody [SV5-Pk1] images:

  Western blot - V5 tag antibody [SV5-Pk1] (ab27671)

Western blot - V5 tag antibody [SV5-Pk1] (ab27671)

Anti-V5 tag antibody [SV5-Pk1] (ab27671) at 1/1000 dilution + mouse cortex cell line lysate at 30 µg

Secondary
Non-Abcam antibody was used: Vector Conjugation: HRP at 1/3000 dilution

Melina Reisenberg, CARD, KCL, London

See Abreview

  Immunocytochemistry/ Immunofluorescence - V5 tag antibody [SV5-Pk1] (ab27671)

Immunocytochemistry/ Immunofluorescence - V5 tag antibody [SV5-Pk1] (ab27671)

ab27671 at a 1/1000 dilution staining V5 tag in CHO cells overexpressing a V5-tagged protein by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton X-100. Samples were blocked with 3% BSA. The secondary used was an Alexa Fluor® 488 conjugated goat anti-mouse IgG (H+L) at a 1/1000 dilution.

Image courtesy of an anonymous Abreview.

References for Anti-V5 tag antibody [SV5-Pk1] (ab27671)

This product has been referenced in:

  • Guo Qet al. Amyloid Precursor Protein Revisited: NEURON-SPECIFIC EXPRESSION AND HIGHLY STABLE NATURE OF SOLUBLE DERIVATIVES. J Biol Chem 287:2437-45 (2012).Read more (PubMed: 22144675) »
  • Thornton JKet al. Differential Subcellular Localization of the Splice Variants of the Zinc Transporter ZnT5 Is Dictated by the Different C-Terminal Regions. PLoS One 6:e23878 (2011). ICC/IF.Read more (PubMed: 21887337) »

See all 7 publications for this product

Publishing research using ab27671? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"