Anti-V5 tag 抗体 [SV5-Pk1] (ab27671)
Key features and details
- Mouse monoclonal [SV5-Pk1] to V5 tag
- Suitable for: Flow Cyt, IHC-Fr, ELISA, IP, WB, RIA
- Reacts with: Species independent
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Anti-V5 tag antibody [SV5-Pk1]
V5 tag 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [SV5-Pk1] to V5 tag -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, IHC-Fr, ELISA, IP, WB, RIAmore details -
種交差性
交差種: Species independent -
免疫原
Tissue, cells or virus corresponding to V5 tag. Paramyxovirus Simian-Virus 5 (SV5)
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エピトープ
ab27671 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. -
ポジティブ・コントロール
- WB: Transfected NIH/3T3 and COS-7 cell lysates.
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特記事項
ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
This antibody was purified from tissue culture supernatant by affinity chromatography. -
一次抗体 備考
Ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope. -
ポリ/モノ
モノクローナル -
クローン名
SV5-Pk1 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27671の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ELISA |
1/1000 - 1/5000.
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IP |
Use at an assay dependent concentration.
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WB | (8) |
1/1000 - 1/5000.
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RIA |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ELISA
1/1000 - 1/5000. |
IP
Use at an assay dependent concentration. |
WB
1/1000 - 1/5000. |
RIA
Use at an assay dependent concentration. |
ターゲット情報
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関連性
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
別名
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
画像
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All lanes : Anti-V5 tag antibody [SV5-Pk1] (ab27671) at 1/1000 dilution
Lanes 1-2 : Transfected NIH/3T3 cell lysate
Lane 3 : Not transfected NIH/3T3 cell lysate
Secondary
All lanes : HRP-conjugated rabbit polyclonal Anti-mouse
Developed using the ECL technique.
Exposure time: 1 secondCells were transfected with a V5-tagged HSPB8 (MW:~25kD) plasmid, which expressed a V5-tagged protein with molecular weight of approximately 28kD.
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Characterization of new C9orf72 dipeptide repeat antibodies.
Rabbit polyclonal antibodies were generated against the sense strand-derived DPRs GA and GR.(A) Western blot of lysates from COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with the indicated vectors. Blots were serially probed with the indicated DPR antibody, then ab27671 and then actin as a loading control.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (129)
ab27671 は 129 報の論文で使用されています。
- Soltanmohammadi N et al. Somatic PMK-1/p38 signaling links environmental stress to germ cell apoptosis and heritable euploidy. Nat Commun 13:701 (2022). PubMed: 35121747
- Wei Y et al. The MYC oncoprotein directly interacts with its chromatin cofactor PNUTS to recruit PP1 phosphatase. Nucleic Acids Res 50:3505-3522 (2022). PubMed: 35244724
- Huo Y et al. The MFN1 and MFN2 mitofusins promote clustering between mitochondria and peroxisomes. Commun Biol 5:423 (2022). PubMed: 35523862
- Song PX et al. Septin 9 and phosphoinositides regulate lysosome localization and their association with lipid droplets. iScience 25:104288 (2022). PubMed: 35573204
- Wang H et al. Nuclear TIGAR mediates an epigenetic and metabolic autoregulatory loop via NRF2 in cancer therapeutic resistance. Acta Pharm Sin B 12:1871-1884 (2022). PubMed: 35847493