Anti-V5 tag antibody [SV5-Pk1] (ab27671)

Thank you very much for your prompt response. Yes, we would like to have one vial of ab27671 (anti-V5) for replacement. Although the Mdr1 antibody did not work well, but I am very satisfied with your help. Have a great day!

ANSWER:

Thank you for your kinds words. I am happy to help!

As requested, I have issued a free of charge replacement for one vial of ab27671.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

We have talked on the phone and e-mailed back and forth about the Mdr1 antibody (please see the e-mails below). We tested the replacement Mdr1 (ab3366) antibody and it still did not work. To make sure that our samples have no problem, we kept trying different Mdr1 antibody from other company. As shown on the attached picture, we did see that Mdr1 level was enhanced for A549 cells after CDDP treatment but not for pc14 cells (the right picture) when detected with one Mdr1 antibody from Santa Cruz. However, when probed with ab3366 for the same membrane, we did not see the specific signal for Mdr1 (the left picture). We would like to request replacement for anti-V5 antibody (ab27671). Hopefully this antibody will work well and I don’t need to keep bothering you. Thank you very much for your help!

ANSWER:

Thanks for your reply. I am sorry to hear the replacement vial of ab3366 did not improve things.

Just to make sure, you would like me to send along one vial of ab27671 as a replacement for this most recent faulty vial of ab3366? If this is your request I can certainly take care of that for you.

Hello, I have a technical question on the specificity of two of your antibody products, a monoclonal and a polyclonal, raised against the V5 peptide tag.   These are: 1) Anti-V5 tag antibody [SV5-Pk1] (ab27671) http://www.abcam.com/V5-tag-antibody-SV5-Pk1-ab27671.html   2) Anti-V5 tag antibody - ChIP Grade (ab9116) http://www.abcam.com/V5-tag-antibody-ChIP-Grade-ab9116.html     We are currently using these antibodies for detection of a recombinant protein which has the V5 tag at the C-terminus.   Did you or someone else test this antibody for crossreactivity with human proteins in different tissues/cell lines? If yes, what were the results?   Thank you very much!  

ANSWER:

For ab27671 we only have data on it being used in HEK cells and for ab9116, it was used in HeLa and the western blot data is available on the public website, in the Abreview section. Both antibodies appeared to give a strong signal in cells transfected with a V5 tagged protein, but did not give a signal in the non-transfected cell lane. If there is anything else I can help you with, please let me know.