Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Tags & Cell Markers >> Epitope Tags >> V5 Tag
Overview
Product name
Anti-V5 tag antibody - ChIP Grade
V5 tag 抗体 (24件) 一覧
Description
Rabbit polyclonal to V5 tag - ChIP Grade
Specificity
ELISA: Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:50,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP- conjugated goat anti rabbit (IgG) at 1:10000 and TMB as the substrate. Appropriate specificity controls were run.
Tested applications
ICC/IF, ChIP, WB, ELISA, IP, ChIP/Chipmore details
Immunogen
Positive control
Ab5395
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg .
Clonality
Polyclonal
Isotype
IgG
Light chain type
unknown
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Tags & Cell Markers >> Epitope Tags >> V5 Tag
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
ChIP: Use at an assay dependent dilution. (PubMed: 17289569)
WB: Use at an assay dependent dilution. Can be blocked with V5 tag peptide (ab15829).
ELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
ChIP/Chip: Use at an assay dependent dilution. (PubMed: 17289569)
Target
Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Cellular localization
Not applicable
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Anti-V5 tag antibody - ChIP Grade images:
Western blot - V5 tag antibody - ChIP Grade (ab9116)
All lanes : Anti-V5 tag antibody - ChIP Grade (ab9116) at 1/5000 dilution
Lane 1 : Cells transfected with v5-tagged protein.
Lane 2 : Cells transfected with v5-tagged protein.
Lane 3 : Cells transfected with v5-tagged protein.
Lane 4 : Untransfected cells.
Lane 5 : Untransfected cells.
Secondary
Lanes 1 - 3 : Anti-rabbit Ig-HRP at 1/20000 dilution
Lanes 4 - 5 : Anti-rabbit Ig-HRP at 1/20000 dilution
Mouse ES cells lysate.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - V5 tag antibody - ChIP Grade (ab9116)
ab9116 at 1/1000 dilution staining V5 in V5-Pgc transfected Drosophila Kc cells by Immunocytochemistry/ Immunofluorescence. Kc cells were fixed in 4% formaldehyde for 10 minutes, washed in PBS-Tween 20 (0.05%) and blocked in 5% milk in PBS-Tween 20 for 1 hour. Cells were incubated with primary antibody at 4°C overnight and the secondary antibody at room temperature for 3 hours.
Image from Timinszky G. et.al., PLoS ONE. 2008; 3(6): e2506 (Fig. 2)
References for Anti-V5 tag antibody - ChIP Grade (ab9116)
This product has been referenced in:
- Duguay Det al. Dynein Light Chain Tctex-Type 1 Modulates Orexin Signaling through Its Interaction with Orexin 1 Receptor. PLoS One 6:e26430 (2011). ELISA.Read more (PubMed: 22028875) »
- Cai Jet al. Diacylglycerol Kinase {delta} and Protein Kinase C{alpha} Modulate Epidermal Growth Factor Receptor Abundance and Degradation through Ubiquitin-specific Protease 8. J Biol Chem 285:6952-9 (2010). IP.Read more (PubMed: 20064931) »
See all 15 publications for this product
Publishing research using ab9116? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"