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Western blot - V5 tag antibody - ChIP Grade (ab9116)
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Immunocytochemistry/ Immunofluorescence - V5 tag antibody - ChIP Grade (ab9116)
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Product Name
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V5 tag antibody - ChIP Grade
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V5 tag 抗体 (23件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to V5 tag - ChIP Grade
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Immunogen
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GKPIPNPLLGLDST (V5 epitope) conjugated with KLH.
BLAST 'GKPIPNPLLGLDST' with  or 
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Specificity
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ELISA: Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:50,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP- conjugated goat anti rabbit (IgG) at 1:10000 and TMB as the substrate. Appropriate specificity controls were run.
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Tested applications
(see key)
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ChIP, ChIP/Chip, ELISA, ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution.
ChIP/Chip: Use at an assay dependent dilution.
ELISA: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent concentration. Can be blocked with V5 tag peptide (ab15829).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Ab5395
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Tags & Cell Markers >> Epitope Tags >> V5 Tag
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Relevance
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The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
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V5 tag antibody - ChIP Grade (ab9116)の別名 |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS, pH 7.2, sodium azide, 0.1% Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg .
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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Abcam では全ての製品情報を一元管理しており、 V5 tag antibody - ChIP Grade について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for V5 tag antibody - ChIP Grade images, references, products related to ab9116 and other tools.
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V5 tag antibody - ChIP Grade images:
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 Western blot - V5 tag antibody - ChIP Grade (ab9116)
All lanes : V5 tag antibody - ChIP Grade (ab9116) at 1/5000 dilution
Lane 1 : Cells transfected with v5-tagged protein.
Lane 2 : Cells transfected with v5-tagged protein.
Lane 3 : Cells transfected with v5-tagged protein.
Lane 4 : Untransfected cells.
Lane 5 : Untransfected cells.
Secondary
Lanes 1 - 3 : Anti-rabbit Ig-HRP at 1/20000 dilution
Lanes 4 - 5 : Anti-rabbit Ig-HRP at 1/20000 dilution
Mouse ES cells lysate. This image is courtesy of an anonymous Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence - V5 tag antibody - ChIP Grade (ab9116)
ab9116 at 1/1000 dilution staining V5 in V5-Pgc transfected Drosophila Kc cells by Immunocytochemistry/ Immunofluorescence. Kc cells were fixed in 4% formaldehyde for 10 minutes, washed in PBS-Tween 20 (0.05%) and blocked in 5% milk in PBS-Tween 20 for 1 hour. Cells were incubated with primary antibody at 4°C overnight and the secondary antibody at room temperature for 3 hours. Image from Timinszky G. et.al., PLoS ONE. 2008; 3(6): e2506 (Fig. 2)
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References for V5 tag antibody - ChIP Grade (ab9116)
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This product has been used in: (two most recent references)
Cai J et al. Diacylglycerol Kinase {delta} and Protein Kinase C{alpha} Modulate Epidermal Growth Factor Receptor Abundance and Degradation through Ubiquitin-specific Protease 8. J Biol Chem 285:6952-9 (2010). IP. PubMed: 20064931
Szuplewski S et al. Drosophila Minus is required for cell proliferation and influences Cyclin E turnover. Genes Dev 23:1998-2003 (2009). WB. PubMed: 19723762
See all 13 publication references for this product.
If you publish research using ab9116 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to V5 tag
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