|
|
|
|
ChIP - V5 tag antibody - ChIP Grade (ab15828)
(enlarge)
|
|
Western blot - V5 tag antibody (ab15828)
(enlarge)
|
|
|
|
|
Product Name
|
V5 tag antibody - ChIP Grade
|
| |
V5 tag 抗体 (22件) 一覧
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to V5 tag - ChIP Grade
|
|
Immunogen
|
Synthetic peptide: GKPIPNPLLGLDST(C) (V5 epitope) conjugated to KLH.
(Antibody raised against peptide available as ab15829.)
|
|
Specificity
|
Specificity was confirmed by Western (see picture) and by peptide ELISA against the immunising peptide.
|
|
Tested applications
(see key)
|
ChIP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ChIP: ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.
WB: Use at a concentration of 0.5 µg/ml. Can be blocked with V5 tag peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Tags & Cell Markers >> Epitope Tags >> V5 Tag
|
|
Relevance
|
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
|
|
|
V5 tag antibody - ChIP Grade (ab15828)の別名 |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
Abcam では全ての製品情報を一元管理しており、 V5 tag antibody - ChIP Grade について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
 |
|
|
See below for V5 tag antibody - ChIP Grade images, references, products related to ab15828 and other tools.
|
| |
V5 tag antibody - ChIP Grade images:
|
 ChIP - V5 tag antibody - ChIP Grade (ab15828)
A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
|
| |
Western blot - V5 tag antibody (ab15828)
All lanes : V5 tag antibody - ChIP Grade (ab15828) at 0.5 µg/ml
Lane 1 : Recombinant V5 tagged Groucho homolog
Lane 2 : Recombinant V5 tagged Groucho with V5 tag peptide (ab15829) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Goat anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
|
| |
References for V5 tag antibody - ChIP Grade (ab15828)
|
|
This product has been used in: (two most recent references)
Cironi L et al. IGF1 is a common target gene of Ewing's sarcoma fusion proteins in mesenchymal progenitor cells. PLoS ONE 3:e2634 (2008). ChIP. PubMed: 18648544
Mizuguchi G et al. Nonhistone Scm3 and Histones CenH3-H4 Assemble the Core of Centromere-Specific Nucleosomes. Cell 129:1153-64 (2007). PubMed: 17574026
If you publish research using ab15828 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to V5 tag
|
| |
V5 tag antibody - ChIP Grade - more information
|
Customer reviews (feedback) regarding V5 tag antibody - ChIP Grade |
Customer FAQs regarding V5 tag antibody - ChIP Grade |
Protocols for V5 tag antibody - ChIP Grade |
Price and availability of products related to V5 tag antibody - ChIP Grade |
| |
V5 tag antibody - ChIP Grade - related products:
|
|
|
| |
V5 tag antibody - ChIP Grade - other tools:
|
Contact Abcam with a Technical Enquiry about V5 tag antibody - ChIP Grade |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
