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Products:Tags & Cell Markers >> Epitope Tags >> V5 Tag
Overview
Product name
Anti-V5 tag antibody - ChIP Grade
V5 tag 抗体 (24件) 一覧
Description
Rabbit polyclonal to V5 tag - ChIP Grade
Specificity
Specificity was confirmed by Western (see image) and by peptide ELISA against the immunising peptide.
Tested applications
ChIP, IP, WBmore details
Immunogen
Synthetic peptide: GKPIPNPLLGLDST(C) (V5 epitope) conjugated to KLH. (Antibody raised against peptide available as ab15829.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Tags & Cell Markers >> Epitope Tags >> V5 Tag
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution. (ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.)
IP: Use at an assay dependent dilution. (PubMed: 20850014)
WB: Use a concentration of 0.5 µg/mlCan be blocked with V5 tag peptide (ab15829).
Target
Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Cellular localization
Not applicable
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Anti-V5 tag antibody - ChIP Grade images:
ChIP - V5 tag antibody - ChIP Grade (ab15828)
A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
Western blot - V5 tag antibody (ab15828)
All lanes : Anti-V5 tag antibody - ChIP Grade (ab15828) at 0.5 µg/ml
Lane 1 : Recombinant V5 tagged Groucho homolog
Lane 2 : Recombinant V5 tagged Groucho with V5 tag peptide (ab15829) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Goat anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
References for Anti-V5 tag antibody - ChIP Grade (ab15828)
This product has been referenced in:
- Zhang Let al. The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor. Genes Dev 25:1262-74 (2011).Read more (PubMed: 21685362) »
- Ding L & Forsburg SL Schizosaccharomyces pombe Minichromosome Maintenance-binding Protein (MCM-BP) Antagonizes MCM Helicase. J Biol Chem 286:32918-30 (2011). IP.Read more (PubMed: 21813639) »
See all 8 publications for this product
Publishing research using ab15828? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"