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ab15829 |
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ab51501 |
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ab52579 |
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ab46666 |
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Read our guarantee »Publishing research using ab15828? Please let us know so that we can cite the reference in this datasheet
ab15828 has been referenced in 8 publications.
Publishing research using ab15828? Please let us know so that we can cite the reference in this datasheet
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A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
All lanes : Anti-V5 tag antibody - ChIP Grade (ab15828) at 0.5 µg/ml
Lane 1 : Recombinant V5 tagged Groucho homolog
Lane 2 : Recombinant V5 tagged Groucho with
Lysates/proteins at 20 µg per lane.
Secondary
Goat anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
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