V5 tag antibody (Agarose) (ab1229)

Product Name 

V5 tag antibody (Agarose)

Product type 

Primary antibodies

Description 

Goat polyclonal to V5 tag (Agarose)

Immunogen 

Full length native GKPIPNPLLGLDST (V5) (purified): conjugated to KLH.
BLAST 'GKPIPNPLLGLDST' with or

Tested applications 
(see key)

IP

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IP: Use at an assay dependent concentration. Use at a concentration of 15 - 20 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Tags & Cell Markers >> Epitope Tags >> Agarose Conjugates
Tags & Cell Markers >> Epitope Tags >> V5 Tag

Relevance 

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Conjugation notes 

Goat anti-V5 affinity-purified antibodies were coupled to agarose beads using a cyanogen bromide method.

Storage buffer 

0.01 M Phosphate, pH 7.2, 0.1 M NaCl, 0.1% Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Conjugation 

Agarose

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C.

Notes 

Provided as 400µl of slurry (200µl gel + 200µl buffer). 100µg of the affinity purified antibody is attached to the gel at a ratio of 500µg antibody/ml of gel or 250µg IgG/ml of 50% slurry.

References for V5 tag antibody (Agarose) (ab1229)

  This product has been used in: (two most recent references)

Vos WL et al. Expression and structural characterization of peripherin/RDS, a membrane protein implicated in photoreceptor outer segment morphology. Eur Biophys J 39:679-88 (2010). IP. PubMed: 19921174

Zhang Z et al. Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells. Proc Natl Acad Sci U S A 101:8882-7 (2004). PubMed: 15187228
If you publish research using ab1229 please let us know so that we can cite the reference on this datasheet.

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