Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Ureter)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Ureter
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/50
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Submitted composite image shows excellent positivity in lumenal cells of the ureter ( upper image) but there is extensive positivity in the Glomeruli and tubules of the kidney( lower image).
I am not familar with this positivity.
Abcam response
Thank you for your valuable feedback. ab93721 has not been previously tested in cow tissue and your data suggests that ab93721 may not be suitable for detecting Uroplakin III on cow tissue using IHC-P under these experimental conditions. We hope this information is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Nov 30 2015