Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
20 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey® Blocker 1:1 in PBS + 0.1% Tween
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor 680 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000
Detection
Detection method
Odyssey® scanner
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 36 kDa Non-specific: 34 kDa
Positive control
Whole Cell Extract
Negative control
-
Additional data
Additional Notes
Picture legend.
1,2 - HeLa Whole Cell extract (20 & 40 µg, respectively)
1,2 - HeLa Whole Cell extract (20 & 40 µg, respectively)
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投稿 Jan 13 2011