HRP Anti-Ubiquinol-Cytochrome C Reductase Core Protein I 抗体 [16D10AD9AH5] (ab197968)
Key features and details
- HRP Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I
- Suitable for: WB, IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5]
Ubiquinol-Cytochrome C Reductase Core Protein I 一次抗体 製品一覧 -
製品の詳細
HRP Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I -
由来種
Mouse -
標識
HRP -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Full length native protein (purified) corresponding to Cow Ubiquinol-Cytochrome C Reductase Core Protein I.
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ポジティブ・コントロール
- WB: Human heart mitochondrial lysate and HepG2 whole cell lysate. IHC-P: Normal human colon tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
ab197968 is produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
16D10AD9AH5 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab197968の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1. -
配列類似性
Belongs to the peptidase M16 family. UQCRC1/QCR1 subfamily. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 282393 Cow
- Entrez Gene: 7384 Human
- Entrez Gene: 22273 Mouse
- Entrez Gene: 301011 Rat
- Omim: 191328 Human
- SwissProt: P31800 Cow
- SwissProt: P31930 Human
- SwissProt: Q8K2S8 Mouse
see all -
別名
- Complex III subunit 1 antibody
- Core protein I antibody
- Cytochrome b c1 complex subunit 1, mitochondrial antibody
see all
画像
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HRP Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab197968) at 1/5000 dilution + Human heart tissue lysate - mitochondrial extract (ab110337) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 18 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197968 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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HRP Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab197968) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197968 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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IHC image of Ubiquinol-Cytochrome C Reductase Core Protein I staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab197968, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab197968 は論文での使用が確認できていません。