Anti-Ube2L3/UBCH7 抗体 [EPR4368] (ab108936)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4368] to Ube2L3/UBCH7
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Ube2L3/UBCH7 antibody [EPR4368]
Ube2L3/UBCH7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4368] to Ube2L3/UBCH7 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa, LNCaP, Jurkat and K562 cell lysates IHC-P: Human testis tissue IF: HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR4368 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108936の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/250.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
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特記事項 |
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ICC/IF
1/100 - 1/250. |
WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
ターゲット情報
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機能
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. -
組織特異性
Ubiquitous, with highest expression in testis. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the ubiquitin-conjugating enzyme family. -
翻訳後修飾
Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 7332 Human
- Entrez Gene: 100042355 Mouse
- Entrez Gene: 22195 Mouse
- Entrez Gene: 363836 Rat
- Omim: 603721 Human
- SwissProt: P68036 Human
- SwissProt: P68037 Mouse
- Unigene: 108104 Human
see all -
別名
- E2 F1 antibody
- L UBC antibody
- L-UBC antibody
see all
画像
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All lanes : Anti-Ube2L3/UBCH7 antibody [EPR4368] (ab108936) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : LNCaP cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 18 kDa -
ab108936, at a 1/100 dilution, staining Ube2L3/UBCH7 in paraffin-embedded Human testis tissue
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab108936, at a 1/100 dilution, staining Ube2L3/UBCH7 in HeLa cells
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab108936 は 5 報の論文で使用されています。
- Yi SA et al. HPV-mediated nuclear export of HP1? drives cervical tumorigenesis by downregulation of p53. Cell Death Differ N/A:N/A (2020). PubMed: 32203172
- Yi SA et al. HP1? Sensitizes Cervical Cancer Cells to Cisplatin through the Suppression of UBE2L3. Int J Mol Sci 21:N/A (2020). PubMed: 32825184
- Liu Y et al. UBE2L3, a susceptibility gene that plays oncogenic role in hepatitis B-related hepatocellular carcinoma. J Viral Hepat N/A:N/A (2018). PubMed: 29969176
- Ma X et al. Ubiquitin conjugating enzyme E2 L3 promoted tumor growth of NSCLC through accelerating p27kip1 ubiquitination and degradation. Oncotarget 8:84193-84203 (2017). PubMed: 29137415
- Wang C et al. HTLV-1 Tax Functions as a Ubiquitin E3 Ligase for Direct IKK Activation via Synthesis of Mixed-Linkage Polyubiquitin Chains. PLoS Pathog 12:e1005584 (2016). PubMed: 27082114