Anti-USP5 抗体 (ab84695)
Key features and details
- Rabbit polyclonal to USP5
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-USP5 antibody
USP5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to USP5 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residue 375 and 425 of Human USP5 (CAA62690.1).
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ポジティブ・コントロール
- IHC: Human Colon Carcinoma, human ovarian carcinoma
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特記事項
Concentration is optimized for IHC and not determined
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84695の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. -
配列類似性
Belongs to the peptidase C19 family.
Contains 2 UBA domains.
Contains 1 UBP-type zinc finger. -
ドメイン
The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. -
翻訳後修飾
The N-terminus is blocked. - Information by UniProt
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参照データベース
- Entrez Gene: 8078 Human
- Entrez Gene: 100174079 Orangutan
- Omim: 601447 Human
- SwissProt: P45974 Human
- SwissProt: Q5R407 Orangutan
- Unigene: 631661 Human
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別名
- Deubiquitinating enzyme 5 antibody
- Isopeptidase T antibody
- ISOT 1 antibody
see all
画像
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ab84695, at 1/100 dilution, staining USP5 in formalin-fixed, paraffin-embedded human colon carcinoma by Immunohistochemistry using a Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.
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ab84695, at 1/100 dilution, staining USP5 in formalin-fixed, paraffin-embedded human colon carcinoma by Immunohistochemistry using DAB staining.
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ab84695, at 1/500 dilution, staining USP5 in formalin-fixed, paraffin-embedded human ovarian carcinoma by Immunohistochemistry using DAB staining.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab84695 は 1 報の論文で使用されています。
- Kategaya L et al. USP7 small-molecule inhibitors interfere with ubiquitin binding. Nature 550:534-538 (2017). PubMed: 29045385