Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Xenopus laevis Cell lysate - whole cell (Egg extracts)
Specification
Egg extracts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/3000
Secondary antibody
Name
Non-Abcam antibody was used: HRP-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Bands
Specific: 120 kDa Non-specific: 48, 40 kDa
Exposure
2 minute(s) and 0 second(s)
Positive control
HeLa lysates
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 22 2014