Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - other (U2OS)
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Rabbit from GE
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: ~150 kDa Non-specific: 90, 75, 50, 37 kDa
Exposure
1 minute(s) and 0 second(s)
Positive control
Our rtPCR data indicate that U2OS cells express Usp40. Using this abcam antibody, we saw a band at the correct size for Usp40 as well as several other bands. The band at the correct size goes away when we use siRNA to knock down Usp40 expression in U2OS cells. Also, when we express tagged mouse Usp40 in U2OS cells, we see an increase in signal at the correct molecular weight. This antibody does have a lot of background but I am convinced that the band at ~150 is Usp40.
Additional data
Additional Notes
ABTRIAL-XBLWG
Lane 1-U20S siCTL(dharmacon) lysate
Lane 2-U20S + siUSP40 (dharmacon) lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 06 2015