Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Non-reduced Denaturing (10%SDS)
Loading amount
80 µg
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%milk
Dilution
1/500
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Fluorescence conjugated secondary antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
Detection
Detection method
Odyssey
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 120 kDa Non-specific: 75 kDa
Additional data
Additional Notes
293T transfected with some siRNAsagainst genes (not USP13) in our study to test the subsequent effects on the expression of USP13 using western blotting.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 08 2016