Anti-FIP 抗体 (ab125184)
Key features and details
- Rabbit polyclonal to FIP
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-FIP antibody
FIP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FIP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Orangutan -
免疫原
Synthetic peptide corresponding to Human FIP aa 296-346.
-
ポジティブ・コントロール
- HeLa, Jurkat and 293T whole cell lysates IHC: Human prostate carcinoma.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125184の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
|
|
IP |
Use at 2-10 µg/mg of lysate.
|
|
IHC-P |
Use a concentration of 1 µg/ml.
|
特記事項 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 37 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
Use a concentration of 1 µg/ml. |
ターゲット情報
-
機能
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. -
組織特異性
Ubiquitous. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 7392 Human
- Omim: 600390 Human
- SwissProt: Q15853 Human
- SwissProt: Q6FI33 Human
- Unigene: 454534 Human
-
別名
- bHLHb12 antibody
- Class B basic helix-loop-helix protein 12 antibody
- FIP antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling FIP with ab125184 at 1 µg/mL. DAB was used as detection. Counterstained with hematoxylin.
-
All lanes : Anti-FIP antibody (ab125184) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 30 seconds -
ab125184, at 6 µg/mg lysate, detecting FIP in 293T cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
For the subsequent Western blot, ab125184 was used at 1 µg/ml.
The blot was developed using the ECL technique, with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab125184 は 4 報の論文で使用されています。
- Liu F et al. USF2 enhances the osteogenic differentiation of PDLCs by promoting ATF4 transcriptional activities. J Periodontal Res 55:68-76 (2020). PubMed: 31448831
- Fang E et al. Therapeutic targeting of YY1/MZF1 axis by MZF1-uPEP inhibits aerobic glycolysis and neuroblastoma progression. Theranostics 10:1555-1571 (2020). PubMed: 32042322
- Khazeem MM et al. Transcription of carbonyl reductase 1 is regulated by DNA topoisomerase II beta. FEBS Lett 594:3395-3405 (2020). PubMed: 32767399
- Schaeffer V et al. Binding of OTULIN to the PUB domain of HOIP controls NF-?B signaling. Mol Cell 54:349-61 (2014). PubMed: 24726327