Anti-UQCRFS1/RISP 抗体 [EPR16289] - C-terminal (ab191079)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16289] to UQCRFS1/RISP - C-terminal
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal
UQCRFS1/RISP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR16289] to UQCRFS1/RISP - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, ICC/IF, IP, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HeLa, Raji, 293, PC12, mouse brain and Human fetal heart lysates; Human kidney tissue; HepG2 and HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16289 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab191079の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/280.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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IP |
1/90.
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WB |
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa).
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特記事項 |
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Flow Cyt (Intra)
1/280. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
IP
1/90. |
WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 30 kDa). |
ターゲット情報
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機能
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex. -
配列類似性
Contains 1 Rieske domain. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7386 Human
- Entrez Gene: 66694 Mouse
- Entrez Gene: 291103 Rat
- Omim: 191327 Human
- SwissProt: P47985 Human
- SwissProt: Q9CR68 Mouse
- SwissProt: P20788 Rat
- Unigene: 726119 Human
see all -
別名
- Complex III subunit 5 antibody
- Complex III subunit IX antibody
- Cytochrome b c1 complex subunit Rieske mitochondrial antibody
see all
画像
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All lanes : Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079) at 1/1000 dilution
Lane 1 : PC12 cell lysate
Lanes 2-3 : Mouse brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa -
Western blot analysis of immunoprecipitation pellet from Human fetal heart lysate immunoprecipitated using ab191079 at 1/90 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling UQCRFS1/RISP with ab191079 at 1/280 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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Immunofluorescent analysis of HepG2 cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100 permeabilized) labeling UQCRFS1/RISP with ab191079 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue). Negative controls also shown.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UQCRFS1/RISP with ab191079 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-UQCRFS1/RISP antibody [EPR16289] - C-terminal (ab191079) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : 293 cell lysate
Lane 4 : Human fetal heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab191079 は 1 報の論文で使用されています。
- Chen XF et al. Effect of puerarin in promoting fatty acid oxidation by increasing mitochondrial oxidative capacity and biogenesis in skeletal muscle in diabetic rats. Nutr Diabetes 8:1 (2018). PubMed: 29330446