Anti-OPRT 抗体 (ab155763)
Key features and details
- Rabbit polyclonal to OPRT
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-OPRT antibody
OPRT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to OPRT -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to Human OPRT aa 217-466.
Database link: P11172 -
ポジティブ・コントロール
- HeLa and HCT116 whole cell lysates; HeLa cells; Human colon cancer tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155763の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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パスウェイ
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2. -
関連疾患
Defects in UMPS are the cause of orotic aciduria type 1 (ORAC1) [MIM:258900]. A disorder of pyrimidine metabolism resulting in megaloblastic anemia and orotic acid crystalluria that is frequently associated with some degree of physical and mental retardation. A minority of cases have additional features, particularly congenital malformations and immune deficiencies. -
配列類似性
In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
In the C-terminal section; belongs to the OMP decarboxylase family. - Information by UniProt
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参照データベース
- Entrez Gene: 281568 Cow
- Entrez Gene: 7372 Human
- Entrez Gene: 22247 Mouse
- Entrez Gene: 288051 Rat
- Omim: 613891 Human
- SwissProt: P31754 Cow
- SwissProt: P11172 Human
- SwissProt: P13439 Mouse
see all -
別名
- ODC antibody
- OMPdecase antibody
- OPRT antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling OPRT with ab155763 at 1/500 dilution.
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Anti-OPRT antibody (ab155763) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells labeling OPRT with ab155763 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab155763 は 1 報の論文で使用されています。
- Suetsugu T et al. Mechanism of acquired 5FU resistance and strategy for overcoming 5FU resistance focusing on 5FU metabolism in colon cancer cell lines. Oncol Rep 45:N/A (2021). PubMed: 33649846