Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (NuPAGE Novex 4-12% Bis-Tris mini gel from Invitrogen)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: 2% milk in PBST
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG (whole molecule), Sigma A4914-1ml
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: about 65 kDa Non-specific: about 15, 25, 45, 55 kDa
Positive control
Mouse liver microsomes
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Xia Wen
Verified customer
投稿 Jul 25 2011