Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Retina, Cryosections)
Antigen retrieval step
None
Permeabilization
Yes - 0,5% Triton-x-100
Specification
Retina, Cryosections
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 3% NGS, 1% BSA, 0,5% Triton-x-100 , PBS
Dilution
1/1000
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: goat-anti-rabbit-488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Picture:
DAPI, anti--mouse CTBP2 (BD-Bioscience, 1/1000 with rabbit-anti-mouse-Cy3 1/500) Ribbon Synapse Marker,
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Lisa Janina Klotz
Verified customer
投稿 Mar 28 2018