Anti-UBC4 抗体 (ab125946)
Key features and details
- Rabbit polyclonal to UBC4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-UBC4 antibody
UBC4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to UBC4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Cow, Pig, Drosophila melanogaster, Zebrafish, Orangutan -
免疫原
Synthetic peptide corresponding to Human UBC4 aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab126283) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; NIH3T3; HEK293; HepG2; MCF7.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125946の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). |
ターゲット情報
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機能
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the ubiquitin-conjugating enzyme family. - Information by UniProt
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参照データベース
- Entrez Gene: 7322 Human
- Entrez Gene: 56550 Mouse
- Entrez Gene: 641452 Rat
- Omim: 602962 Human
- SwissProt: P62837 Human
- SwissProt: P62838 Mouse
- SwissProt: Q06AA9 Pig
- SwissProt: P62839 Rat
see all -
別名
- E2(17)KB2 antibody
- OTTHUMP00000223475 antibody
- OTTHUMP00000223476 antibody
see all
画像
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All lanes : Anti-UBC4 antibody (ab125946) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 16 kDa
Additional bands at: 102 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab125946 は論文での使用が確認できていません。