Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH-3T3)
Permeabilization
Yes - 0.2% Triton X-100
Specification
NIH-3T3
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 10% goat serum 5% BSA
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: CY 5 goat anti-rat IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Spencer Goodman
Verified customer
投稿 May 23 2017