Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain, Raphe Nucleus)
Permeabilization
Yes - Triton X-100 0.1%
Specification
Brain, Raphe Nucleus
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking solution
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: R & D Systems NorthernLights NL493
Host species: Rabbit
Clonality: Polyclonal
Host species: Rabbit
Clonality: Polyclonal
Additional data
Additional Notes
We received a very weak signal from this Ab, indistinguishable from the background.
Abcam response
Thank you for your valuable feedback and for participating in our Abreview testing discount program. We are sorry to hear that this antibody has not given you good results in immunohistochemistry using mouse frozen sections. This antibody has not been tested with this application before, and it is possible that it may not be suitable for use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 26 2012