Application
Western blot
Sample
Mouse Cell lysate - whole cell (N1E-115 mouse neuroblastoma)
Loading amount
20 µg
Specification
N1E-115 mouse neuroblastoma
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 20% blocking solution in PBS-T
Secondary antibody
Name
Non-Abcam antibody was used: DAKO
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL Prime
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 40 kDa
Additional data
Additional Notes
Whole cell extract of N1E-115 mouse neuroblastoma cells was used to detect endogenous Tropomodulin 2. This antibody works great with limited background signal, even at long exposures.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 25 2013