Application
Western blot
Sample
Human Cell lysate - whole cell (neuronal cells)
Loading amount
15 µg
Specification
neuronal cells
Treatment
see below (Additional notes)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gels)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (5%BSA in TBST)
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
chemiluminescence
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 145 kDa
Positive control
TrkA protein
Additional data
Additional Notes
lanes:
neuronal cells:
1 = untreated
2 = treated with rh-ßNGF 25ng/ml
3 = treated with rh-ßNGF 50ng/ml
4 = treated with rh-NT3 50ng/ml
5 = treated with rh-BDNF 50ng/ml
6 = positive control: TrkA protein
neuronal cells:
1 = untreated
2 = treated with rh-ßNGF 25ng/ml
3 = treated with rh-ßNGF 50ng/ml
4 = treated with rh-NT3 50ng/ml
5 = treated with rh-BDNF 50ng/ml
6 = positive control: TrkA protein
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 09 2012