Anti-Triosephosphate isomerase 抗体 (ab96696)
Key features and details
- Rabbit polyclonal to Triosephosphate isomerase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Triosephosphate isomerase antibody
Triosephosphate isomerase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Triosephosphate isomerase -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to a region within amino acids 187-249 of Human Triosephosphate isomerase (NP_000356).
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ポジティブ・コントロール
- WB: 293T, HeLaS3, HepG2 and Raji cell lysates; A431, H1299, whole cell lysates. IHC-P: SNU16 tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96696の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 27 kDa.
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IHC-P |
1/100.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 27 kDa. |
IHC-P
1/100. |
ターゲット情報
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関連性
Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. -
細胞内局在
Cytoplasmic and Nuclear; extracellular vesicle exosome; extracellular space. -
参照データベース
- Entrez Gene: 7167 Human
- Omim: 190450 Human
- SwissProt: P60174 Human
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別名
- epididymis secretory protein Li 49 antibody
- HEL-S-49 antibody
- MGC88108 antibody
see all
画像
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All lanes : Anti-Triosephosphate isomerase antibody (ab96696) at 1/2000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE -
ab96696, at a 1/100 dilution, staining Triosephosphate isomerase in paraffin embedded SNU16 (human) by Immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab96696 は 8 報の論文で使用されています。
- Coulibaly A et al. STAT3 governs the HIF-1a response in IL-15 primed human NK cells. Sci Rep 11:7023 (2021). PubMed: 33782423
- Wu L et al. Niche-Selective Inhibition of Pathogenic Th17 Cells by Targeting Metabolic Redundancy. Cell 182:641-654.e20 (2020). PubMed: 32615085
- Velásquez SY et al. Innate Cytokine Induced Early Release of IFN? and CC Chemokines from Hypoxic Human NK Cells Is Independent of Glucose. Cells 9:N/A (2020). PubMed: 32192004
- Gao W et al. Targeting oxidative pentose phosphate pathway prevents recurrence in mutant Kras colorectal carcinomas. PLoS Biol 17:e3000425 (2019). PubMed: 31461438
- Lone SN et al. Triose-phosphate isomerase is a novel target of miR-22 and miR-28, with implications in tumorigenesis. J Cell Physiol 233:8919-8929 (2018). PubMed: 29856481
- Rai V et al. Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae. Sci Rep 7:45732 (2017). PubMed: 28378827
- Peng X et al. Autophagy promotes paclitaxel resistance of cervical cancer cells: involvement of Warburg effect activated hypoxia-induced factor 1-a-mediated signaling. Cell Death Dis 5:e1367 (2014). WB ; Human . PubMed: 25118927
- Mukai C et al. Biomimicry enhances sequential reactions of tethered glycolytic enzymes, TPI and GAPDHS. PLoS One 8:e61434 (2013). WB ; Mouse . PubMed: 23626684