Application
Western blot
Sample
Mouse Serum (Mouse Serum)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Mouse Serum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 27°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Li-Cor Goat anti Rabbit Alexa 800
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 790
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 790
Detection
Detection method
Li-Cor Scanner
Exposure
3 minute(s) and 0 second(s)
Positive control
Li-Cor Goat anti-Mouse Alexa 680 detects 25 KD IgG light chain
Additional data
Additional Notes
The antibody is raised again human TFF3 protein, here we just verified that it is not working with mouse TFF3.
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with mouse and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jul 28 2016