Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK cells)
Permeabilization
Yes - 0.1% DOC
Specification
MDCK cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
It has been known that the incubation of MDCK cells at 20 C for 2h delays the protein traffic from trans Golgi. After the incubation at low temprature, the accumulation of TfR proteins is observed in trans Golgi.
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馨 宮原
Verified customer
投稿 Oct 23 2015