Blocking step: Milk as blocking agent for 1 hour · Concentration: 5 % · Temperature: RT°C
Dilution: 1/100
Incubation time: 13 hours Temperature: 4°C Diluent: 1x TBS with 0.1% Blocking
Secondary antibody
Name: anti-mouse Conjugation: HRP
Dilution:1/200
Detection method: ECL chemi method
Dilution: 1/100
Incubation time: 13 hours Temperature: 4°C Diluent: 1x TBS with 0.1% Blocking
Secondary antibody
Name: anti-mouse Conjugation: HRP
Dilution:1/200
Detection method: ECL chemi method
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投稿 Nov 01 2017