MitoProfile Total OXPHOS
40 ug total protein from human heart tissue whole lysate
12% tris-glycine gel
5% milk-TBST block, 1 h, RT
primary of 1:750 in 5% milk for 16 h at 4C
secondary of 1:1250 in 5% milk for 1 h at RT
exposure varied based on band
excellent ease of use - 5/5
image attached
40 ug total protein from human heart tissue whole lysate
12% tris-glycine gel
5% milk-TBST block, 1 h, RT
primary of 1:750 in 5% milk for 16 h at 4C
secondary of 1:1250 in 5% milk for 1 h at RT
exposure varied based on band
excellent ease of use - 5/5
image attached
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投稿 Oct 29 2014