Application
Western blot
Sample
Mouse Tissue lysate - whole (HEK and Mouse and Rat hippocapus)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HEK and Mouse and Rat hippocapus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TTBS
Concentration
1 µg/ml
Secondary antibody
Name
Non-Abcam antibody was used: Mouse IgG HRP Linked Whole Ab
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: 22 kDa
Additional data
Abcam response
Thank you for your valuable feedback. We have not previously tested this antibody in Western Blot and therefore it may not be suitable for use in this application. Further optimization may improve results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 03 2017