Anti-PAR1/Thrombin Receptor 抗体 (ab32611)
Key features and details
- Rabbit polyclonal to PAR1/Thrombin Receptor
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-PAR1/Thrombin Receptor antibody
PAR1/Thrombin Receptor 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PAR1/Thrombin Receptor -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Baboon -
免疫原
-
ポジティブ・コントロール
- KNRK cells, Pancreatic tissue.
-
特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32611の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).
|
|
IHC-P |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
特記事項 |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
-
機能
High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development. -
組織特異性
Platelets and vascular endothelial cells. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
翻訳後修飾
A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor. -
細胞内局在
Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 2149 Human
- Omim: 187930 Human
- SwissProt: P56488 Baboon
- SwissProt: P25116 Human
- Unigene: 482562 Human
-
別名
- CF2R antibody
- Coagulation factor II (thrombin) receptor antibody
- Coagulation factor II receptor antibody
see all
画像
-
Anti-PAR1/Thrombin Receptor antibody (ab32611) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 87 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesPAR1/Thrombin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
-
This image shows human pancreas stained with ab32611.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (13)
ab32611 は 13 報の論文で使用されています。
- Guan Y et al. Protease-Activated Receptor 1 Contributes to Microcirculation Failure and Tubular Damage in Renal Ischemia-Reperfusion Injury in Mice. Biomed Res Int 2021:6665714 (2021). PubMed: 33681367
- Dekker NAM et al. Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental cardiopulmonary bypass. Intensive Care Med Exp 9:30 (2021). PubMed: 34169407
- Gerasimov A et al. Markers for neural degeneration and regeneration: novel highly sensitive methods for the measurement of thrombin and activated protein C in human cerebrospinal fluid. Neural Regen Res 16:2086-2092 (2021). PubMed: 33642398
- Perry EA et al. Tetracyclines promote survival and fitness in mitochondrial disease models. Nat Metab 3:33-42 (2021). PubMed: 33462515
- Shavit-Stein E et al. Brain Protease Activated Receptor 1 Pathway: A Therapeutic Target in the Superoxide Dismutase 1 (SOD1) Mouse Model of Amyotrophic Lateral Sclerosis. Int J Mol Sci 21:N/A (2020). PubMed: 32408605