Thrombin Activity Assay Kit (Fluorometric) (ab197006)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Plasma, Purified protein
- Sensitivity: 1 ng
製品の概要
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製品名
Thrombin Activity Assay Kit (Fluorometric)
Thrombin キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Plasma, Purified protein -
アッセイタイプ
Quantitative -
検出感度
1 ng -
種交差性
交差種: Mammals, Other species -
製品の概要
Thrombin Activity Assay Kit (Fluorometric) (ab197006) is simple, rapid and can detect Thrombin activity as low as 1 ng in samples.
In the thrombin activity assay protocol, a synthetic AMC-based peptide substrate is proteolytically cleaved by thrombin to release a fluorophore, AMC, which can be easily quantified by fluorescence reader at Ex/Em = 350/450 nm.
Thrombin activity assay protocol summary:
- add standards and samples to wells
- add reaction mix
- analyze with a microplate reader for 30-60 min -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K373 Thrombin Activity Fluorometric Assay Kit. K373-100 is the same size as the 100 test size of ab197006.
Thrombin enzyme (Factor IIa, EC 3.4.21.5), a serine protease, is an important clotting factor in the coagulation cascade that involves the conversion of soluble fibrinogen to insoluble active fibrin strands.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Thrombin Assay Buffer 1 x 15ml Thrombin Dilution Buffer 1 x 1ml Thrombin Standard 1 x 5µl Thrombin Substrate 1 x 0.5ml -
研究分野
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機能
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Factor II deficiency
Ischemic stroke
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2 -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
翻訳後修飾
The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). -
細胞内局在
Secreted, extracellular space. - Information by UniProt
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別名
- Coagulation factor II
- Coagulation factor II thrombin
- EC 3.4.21.5
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab197006 は 5 報の論文で使用されています。
- Meng S et al. MicroRNA-363-3p/sphingosine-1-phosphate receptor 1 axis inhibits sepsis-induced acute lung injury via the inactivation of nuclear factor kappa-B ligand signaling. Exp Anim 71:305-315 (2022). PubMed: 35173110
- Namachivayam K et al. Targeted inhibition of thrombin attenuates murine neonatal necrotizing enterocolitis. Proc Natl Acad Sci U S A 117:10958-10969 (2020). PubMed: 32366656
- Pather K & Augustine TN Tamoxifen induces hypercoagulation and alterations in ERa and ERß dependent on breast cancer sub-phenotype ex vivo. Sci Rep 10:19256 (2020). PubMed: 33159119
- Shefa AA et al. Enhancement of hemostatic property of plant derived oxidized nanocellulose-silk fibroin based scaffolds by thrombin loading. Carbohydr Polym 208:168-179 (2019). PubMed: 30658788
- He P et al. Timely and large dose of clotting factor IX provides better joint wound healing after hemarthrosis in hemophilia B mice. Int J Hematol 110:59-68 (2019). PubMed: 31006077