Anti-Thiophosphate ester 抗体 [51-8] (ab92570)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [51-8] to Thiophosphate ester
- Suitable for: WB
- Reacts with: Species independent
Related conjugates and formulations
製品の概要
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製品名
Anti-Thiophosphate ester antibody [51-8]
Thiophosphate ester 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [51-8] to Thiophosphate ester -
由来種
Rabbit -
特異性
This antibody is specific to thiophosphorylated amino acids. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Thiophosphate ester conjugated to keyhole limpet haemocyanin.
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特記事項
This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Kevan Shokat at University of California at San Francisco.
It is possible to use ab92570 with ab138911 as a kinase reaction reagent for the identification of direct kinase substrates as mentioned in Allen JJ et al. Nat Methods 4:511-6 (2007).
After the kinase of interest has accepted ATP-γ-S (ab138911), p-Nitrobenzyl mesylate (ab138910) can be used to alkylate the thiophospholyation site on the substrates. ab92570 is introduced to identify the tagged substrates.This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
一次抗体 備考
ab92570 can be used to detect kinase substrates containing thiophosphate esters following the kinase reaction as described by J. Allen and colleagues in Nature methods 2007. This antibody as allow researchers to follow this approach to study kinase-substrate relationships of interest. The antibody is also context independent in that it can recognize serine, threonine, and tyrosine thiophosphorylated residues in a multitude of kinase phosphorylation consensus motifs.
In order for this antibody to work in WB and IP, the customer needs to perform a specific kinase reaction using ATP gamma S and PNBM.
See specific Protocol at the bottom of this page for details. -
ポリ/モノ
モノクローナル -
クローン名
51-8 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92570の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000.
|
特記事項 |
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WB
1/5000. |
ターゲット情報
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関連性
Thiophosphate ester is used as fungicide.
画像
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All lanes : Anti-Thiophosphate ester antibody [51-8] (ab92570) at 1/5000 dilution
Lane 1 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug)
Lane 2 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using alkylation with PNBM (2.5mM).
Lane 3 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S
Lane 4 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S followed by alkylation with PNBM (2.5mM).
In vitro kinase reaction was carried out for 30 minutes at room temperature. PNBM alkylation (2.5mM) was for 2 hours at room temperature. Reaction products were analyzed by western blot with thiophosphate ester specific ab92570 at 1/5000, alpha-GST antibody confirms equal loading.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (71)
ab92570 は 71 報の論文で使用されています。
- Roşianu F et al. Loss of NDR1/2 kinases impairs endomembrane trafficking and autophagy leading to neurodegeneration. Life Sci Alliance 6:N/A (2023). PubMed: 36446521
- Franz A et al. Branch point strength controls species-specific CAMK2B alternative splicing and regulates LTP. Life Sci Alliance 6:N/A (2023). PubMed: 36543542
- Bao L et al. A PSTAIRE-type cyclin-dependent kinase controls light responses in land plants. Sci Adv 8:eabk2116 (2022). PubMed: 35089781
- Tavares S et al. FER regulates endosomal recycling and is a predictor for adjuvant taxane benefit in breast cancer. Cell Rep 39:110584 (2022). PubMed: 35385742
- Guo X et al. Dichotomy of the BSL phosphatase signaling spatially regulates MAPK components in stomatal fate determination. Nat Commun 13:2438 (2022). PubMed: 35508457