Testosterone ELISA Kit (ab108666)
Key features and details
- Sensitivity: 0.07 ng/ml
- Range: 0.2 ng/ml - 16 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
製品の概要
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製品名
Testosterone ELISA Kit
Testosterone キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Control Sera 48 <=5.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Control Sera 48 <=10.5% -
サンプルの種類
Urine, Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Competitive -
検出感度
= 0.07 ng/ml -
検出範囲
0.2 ng/ml - 16 ng/ml -
添加回収試験
= 105.5 %
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ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Testosterone ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of Testosterone in serum and plasma.
A 96-well plate has been precoated with anti-Testosterone antibodies. Samples and the Testosterone-HRP conjugate are added to the wells, where Testosterone in the sample competes with the added Testosterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Wash Buffer 1 x 50ml Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit Testosterone conjugate 1 x 12ml Testosterone Control A 1 x 1ml Testosterone Control B 1 x 1ml Testosterone IgG Coated Microtiter Plate (12 x 8 wells) 1 unit Testosterone Standard 0 - 0.0 ng/mL 1 x 1ml Testosterone Standard 1 - 0.6 ng/mL 1 x 1ml Testosterone Standard 2 - 1.4 ng/mL 1 x 1ml Testosterone Standard 3 - 4.0 ng/mL 1 x 1ml Testosterone Standard 4 - 16.0 ng/mL 1 x 1ml TMB Substrate Solution 1 x 15ml -
研究分野
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別名
- 17ß-hydroxyandrost-4-ene-3-one
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参照データベース
- SwissProt: P04278 Human
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (52)
ab108666 は 52 報の論文で使用されています。
- Kelava I et al. Androgens increase excitatory neurogenic potential in human brain organoids. Nature 602:112-116 (2022). PubMed: 35046577
- Chi A et al. ICA II Alleviates Testicular Torsion Injury by Dampening the Oxidative and Inflammatory Stress. Front Endocrinol (Lausanne) 13:871548 (2022). PubMed: 35634492
- Wen M et al. CTBP1‑AS upregulation is associated with polycystic ovary syndrome and can be effectively downregulated by cryptotanshinone. Mol Med Rep 26:N/A (2022). PubMed: 35656892
- Zheng B et al. Testosterone protects mice against zika virus infection and suppresses the inflammatory response in the brain. iScience 25:105300 (2022). PubMed: 36304103
- El-Khadragy MF et al. Chlorogenic acid abates male reproductive dysfunction in arsenic-exposed mice via attenuation of testicular oxido-inflammatory stress and apoptotic responses. Chem Biol Interact 333:109333 (2021). PubMed: 33242462