Application
Immunohistochemistry (Frozen sections)
Sample
Cow Tissue sections (Tendon)
Permeabilization
No
Specification
Tendon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/250
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Submitted image shows that positivity is limited to the peritendineum ( between three negative tendon fascicles).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Sep 21 2015