Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tonsil)
Specification
tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9.0, 20 min, 100C
Permeabilization
No
Other product details
Dilution
1/800
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: dako diluent, background reducers
Secondary antibody
Name
Non-Abcam antibody was used: antimouose anti-rabbit IgG, Leica Refine
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
multiple antigen retrieval regimes tested with EDTA giving highest signal to background, with optimal dilution 1:800
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投稿 Oct 12 2011