Application
ELISA
Sample
Mouse Tissue sections (Cortex protein lysate)
Specification
Cortex protein lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 18°C
Type
Sandwich (Detection)
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 18°C
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
This antibody was tested as the detection antibody in a sELISA test done to asses the levels of S-396-phosphorylated tau protein in the protein lysate from the brain of a WT strain as well as a transgenic mouse strain known to express higher levels of phosphorylated tau.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Eitan Okun
Verified customer
投稿 Aug 23 2016