Anti-Talin 2 抗体 [EPR2536(2)] (ab108967)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2536(2)] to Talin 2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, African green monkey
Related conjugates and formulations
製品の概要
-
製品名
Anti-Talin 2 antibody [EPR2536(2)]
Talin 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2536(2)] to Talin 2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human, African green monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- COS-1, 293T, HeLa, and Jurkat whole cell lysate (ab7899)
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2536(2) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108967の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 270 kDa (predicted molecular weight: 272 kDa).
|
特記事項 |
---|
WB
1/10000 - 1/50000. Detects a band of approximately 270 kDa (predicted molecular weight: 272 kDa). |
ターゲット情報
-
機能
As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity. -
配列類似性
Contains 1 FERM domain.
Contains 1 I/LWEQ domain. -
細胞内局在
Cell junction > focal adhesion. Cell junction > synapse. Cell membrane. Cytoplasm > cytoskeleton. Focal adhesion plaques and synapses. - Information by UniProt
-
参照データベース
- Entrez Gene: 83660 Human
- Entrez Gene: 70549 Mouse
- Entrez Gene: 315776 Rat
- Omim: 607349 Human
- SwissProt: Q9Y4G6 Human
- SwissProt: Q71LX4 Mouse
- Unigene: 569438 Human
- Unigene: 610975 Human
see all -
別名
- DKFZp451B1011 antibody
- DKFZp686I0976 antibody
- DKFZp686K0979 antibody
see all
画像
-
All lanes : Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (purified)
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 272 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (purified) + Jurkat cell lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 272 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (purified) + COS-1 cell lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 272 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
All lanes : Anti-Talin 2 antibody [EPR2536(2)] (ab108967) at 1/50000 dilution (unpurified)
Lane 1 : COS-1 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 272 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab108967 は 3 報の論文で使用されています。
- Wu Y et al. Long Noncoding RNA Lnc-TLN2-4:1 Suppresses Gastric Cancer Metastasis and Is Associated with Patient Survival. J Oncol 2020:8681361 (2020). PubMed: 32256587
- van de Klundert MA et al. Hepatitis B Virus Protein X Induces Degradation of Talin-1. Viruses 8:N/A (2016). WB ; Human . PubMed: 27775586
- Formosa A et al. DNA methylation silences miR-132 in prostate cancer. Oncogene : (2012). PubMed: 22310291