Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (EA hy 926)
Specification
EA hy 926
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1%
Other product details
Dilution
1/100
Incubation time
30 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Additional Notes
Fixed with 1% paraformaldehyde/PBS for 5-10 minutes. Permeabilized with 0.2% Triton X-100 for 5 minutes following 4X PBS rinses. Used Molecular Probes Slow Fade Anti-fad kit to help with visualization.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Adam Szadkowski
Verified customer
投稿 Jan 05 2006