Anti-TXNRD1 抗体 (ab113904)
Key features and details
- Rabbit polyclonal to TXNRD1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TXNRD1 antibody
TXNRD1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TXNRD1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in Human Testis and Spleen tissue lysates as well as the following whole cell lysates: HeLa; HepG2; HEK293; MCF7.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113904の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 71 kDa). Abcam recommends using milk as the blocking agent (3%).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 71 kDa). Abcam recommends using milk as the blocking agent (3%). |
ターゲット情報
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機能
Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid. -
組織特異性
Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver. -
配列類似性
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Contains 1 glutaredoxin domain. -
ドメイン
The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays. -
翻訳後修飾
The N-terminus of isoform 5 is blocked.
ISGylated. -
細胞内局在
Cytoplasm and Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 282388 Cow
- Entrez Gene: 7296 Human
- Entrez Gene: 396681 Pig
- Omim: 601112 Human
- SwissProt: O62768 Cow
- SwissProt: Q16881 Human
- SwissProt: Q9MYY8 Pig
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別名
- cytoplasmic antibody
- Gene associated with retinoic and IFN-induced mortality 12 protein antibody
- Gene associated with retinoic and interferon-induced mortality 12 protein antibody
see all
画像
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All lanes : Anti-TXNRD1 antibody (ab113904) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa, 37 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The predicted molecular weight of TXNRD1 is 71 kDa (SwissProt), however we expect to observe a banding pattern around 60 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab113904 は論文での使用が確認できていません。