Anti-TRP14 抗体 (ab121725)
Key features and details
- Rabbit polyclonal to TRP14
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TRP14 antibody -
製品の詳細
Rabbit polyclonal to TRP14 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human TRP14 aa 1-62.
Sequence:MARYEEVSVSGFEEFHRAVEQHNGKTIFAYFTGSKDAGGKSWCPDCVQAE PVVREGLKHISE
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ポジティブ・コントロール
- IHC-P: Human cerebellum tissue; WB: RT4 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121725の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Disulfide reductase. May participate in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyze dithiol-disulfide exchange reactions. Modulates TNF-alpha signaling and NF-kappa-B activation. Has peroxidase activity and may contribute to the elimination of cellular hydrogen peroxide. -
組織特異性
Ubiquitously expressed in cell lines. -
配列類似性
Belongs to the thioredoxin family.
Contains 1 thioredoxin domain. -
翻訳後修飾
The oxidized protein is reduced by TRXR1. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 84817 Human
- SwissProt: Q9BRA2 Human
- Unigene: 408236 Human
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別名
- 14 kDa thioredoxin-related protein antibody
- Protein 42-9-9 antibody
- Thioredoxin domain-containing protein 17 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling TRP14 with ab121725 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-TRP14 antibody (ab121725) at 0.4 µg/ml + RT4 (human urinary bladder cancer cell line) whole cell lysate
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab121725 は 3 報の論文で使用されています。
- Olivieri M et al. A Genetic Map of the Response to DNA Damage in Human Cells. Cell 182:481-496.e21 (2020). PubMed: 32649862
- Tan WX et al. TRP14 promotes resistance to cisplatin by inducing autophagy in ovarian cancer. Oncol Rep N/A:N/A (2019). PubMed: 31524236
- Zhang SF et al. TXNDC17 promotes paclitaxel resistance via inducing autophagy in ovarian cancer. Autophagy 11:225-38 (2015). PubMed: 25607466