Anti-TULP1 抗体 (ab97281)
Key features and details
- Rabbit polyclonal to TULP1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TULP1 antibody -
製品の詳細
Rabbit polyclonal to TULP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment containing a sequence corresponding to a region within amino acids 281-520 of Human TULP1 (NP_003313).
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ポジティブ・コントロール
- 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji whole cell lysates; Breast CA
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97281の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
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IHC-P |
1/100 - 1/250.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
IHC-P
1/100 - 1/250. |
ターゲット情報
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機能
Required for normal development of photoreceptor synapses. Required for normal photoreceptor function and for long-term survival of photoreceptor cells. Interacts with cytoskeleton proteins and may play a role in protein transport in photoreceptor cells (By similarity). Binds lipids, especially phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, phosphatidylinositol 3,4-bisphosphate, phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-bisphosphate, phosphatidylserine and phosphatidic acid (in vitro). Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages. -
組織特異性
Retina-specific. -
関連疾患
Defects in TULP1 are the cause of retinitis pigmentosa type 14 (RP14) [MIM:600132]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP14 inheritance is autosomal recessive.
Defects in TULP1 are the cause of Leber congenital amaurosis type 15 (LCA15) [MIM:613843]. LCA15 is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. -
配列類似性
Belongs to the TUB family. -
細胞内局在
Cytoplasm. Cell membrane. Secreted. Cell junction > synapse. Detected at synapses between photoreceptor cells and second-order neurons. Does not have a cleavable signal peptide and is secreted by an alternative pathway. - Information by UniProt
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参照データベース
- Entrez Gene: 7287 Human
- Entrez Gene: 22157 Mouse
- Entrez Gene: 309900 Rat
- Omim: 602280 Human
- SwissProt: O00294 Human
- SwissProt: Q9Z273 Mouse
- SwissProt: D3Z809 Rat
- Unigene: 485208 Human
see all -
別名
- RP14 antibody
- Tubby like protein 1 antibody
- Tubby related protein 1 (Tubby like protein 1) antibody
see all
画像
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All lanes : Anti-TULP1 antibody (ab97281) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : Molt-4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of TULP1 in paraffin-embedded Breast CA, using ab97281 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab97281 は 2 報の論文で使用されています。
- Kerek EM et al. A conserved acetylation switch enables pharmacological control of tubby-like protein stability. J Biol Chem N/A:N/A (2020). PubMed: 33187986
- Rodriguez AS et al. Tubby-like protein 1 (Tulp1) is a target of microRNA-134 and is down-regulated in experimental epilepsy. Int J Physiol Pathophysiol Pharmacol 9:178-187 (2017). WB, IHC-P . PubMed: 29348794