Application
Western blot
Sample
Mouse Cell lysate - whole cell (MLE12 cells)
Loading amount
20 µg
Specification
MLE12 cells
Treatment
TTF-1 Plasmid (2ug)
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: IRDye680LT Anti-Rabbit (LICOR)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Dilution
1/20000
Detection
Detection method
Fluorescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 38, 39 kDa
Additional data
Additional Notes
Lane 1 = untreated
Lane 2 = transfection reagent alone
Lane 3 = TTF-1 Plasmid transfection
Lane 2 = transfection reagent alone
Lane 3 = TTF-1 Plasmid transfection
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 20 2013