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- Secondary antibodies
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Product name
Anti-TRPV4 antibody
TRPV4 抗体 (12件) 一覧
Description
Rabbit polyclonal to TRPV4
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide derived from the cytoplasmic n-terminus conserved in mouse, human and rat TRPV4 conjugated to immunogenic carrier protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Sensory System >> Somatosensory system >> Nociception
Neuroscience >> Neurotransmission >> Receptors / Channels >> More Ion Channels
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Western blot - TRPV4 antibody (ab63003)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/300 - 1/3000. Predicted molecular weight: 98 kDa.
IHC-P: 1/300 - 1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr
IHC-Fr: 1/300 - 1/3000.IHC-Fr: 1/300 - 1/3000.
Target
Relevance
The detection of noxious stimuli (chemical, mechanical, or thermal) occurs predominantly at the peripheral terminals of primary afferent neurons. This information is ultimately transmitted to the central nervous system to evoke appropriate protective reflexes. TRPV4 is a non selective calcium permeant, swell activated, cation channel probably involved in osmotic and mechano sensitivity. Activation by exposure to hypotonicity within the physiological range, low pH, citrate and phorbol esters exhibits an outward rectification. Once activated the channel seems to be regulated in a calmodulin dependent manner, with a negative feedback mechanism.
Cellular localization
Multi-pass membrane protein
Alternative names
- osm-9-like TRP channel 4 antibody
- OTRPC 4 antibody
- OTRPC4 antibody
see all
Database links
- Entrez Gene: 59341 Human
- Entrez Gene: 63873 Mouse
- Entrez Gene: 66026 Rat
- Omim: 605427 Human
- SwissProt: Q9HBA0 Human
- SwissProt: Q9EPK8 Mouse
- SwissProt: Q9ERZ8 Rat
- Unigene: 506713 Human
see all
Anti-TRPV4 antibody images:
Western blot - TRPV4 antibody (ab63003)
All lanes : Anti-TRPV4 antibody (ab63003) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 98 kDa
Observed band size : 98 kDa
Additional bands at : 36 kDa,50 kDa,71 kDa. We are unsure as to the identity of these extra bands.
References for Anti-TRPV4 antibody (ab63003)
ab63003 has not yet been referenced specifically in any publications.
Publishing research using ab63003? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"