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Products:Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
Overview
Product name
Anti-TROY antibody
TROY 抗体 (3件) 一覧
Description
Rabbit polyclonal to TROY
Specificity
ab12126 reacts with TROY.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide, corresponding to amino acids 29-44, 204-219, and 409-421 of Human TROY.
Positive control
T98G cell extracts.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Growth factors >> TNF
Signal Transduction >> Growth Factors/Hormones >> TNF
Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 46 kDa (predicted molecular weight: 50.8 kDa).(For optimal results, primary antibody incubations should be performed at room temperature.)
Target
Relevance
TROY is a member of the TNF receptor superfamily. This receptor is highly expressed during embryonic development. It has been shown to interact with TRAF family members, and to activate the JNK signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Cellular localization
Type I membrane protein (Probable).
Alternative names
- TAJ alpha antibody
- TAJ antibody
- TNFRSF 19 antibody
see all
Database links
- Entrez Gene: 55504 Human
- Entrez Gene: 29820 Mouse
- Omim: 606122 Human
- SwissProt: Q9NS68 Human
- SwissProt: Q9JLL3 Mouse
- Unigene: 334174 Human
- Unigene: 281356 Mouse
Anti-TROY antibody images:
Western blot - TROY antibody (ab12126)
Predicted band size : 50.8 kDa
TROY detection by Western blot. The analysis of human TROY using this antibody in T98G, a human glioblastoma cell line extract. A protein band of approximately 46 kDa should be detected.
Immunocytochemistry/ Immunofluorescence - Anti-TROY antibody (ab12126)
ICC/IF image of ab12126 stained Mouse embryonic stem cells cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12126, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-TROY antibody (ab12126)
This product has been referenced in:
- Wang Yet al. Targeted proteasome inhibition by Velcade induces apoptosis in human mesothelioma and breast cancer cell lines. Cancer Chemother Pharmacol 66:455-66 (2010). WB; Human.Read more (PubMed: 19960346) »
- Paulino VMet al. TROY (TNFRSF19) is overexpressed in advanced glial tumors and promotes glioblastoma cell invasion via Pyk2-Rac1 signaling. Mol Cancer Res 8:1558-67 (2010). WB; Human.Read more (PubMed: 20881009) »
See all 2 publications for this product
Publishing research using ab12126? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"