Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Prostate)
Permeabilization
No
Specification
Prostate
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Methanol
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: Blocking agent
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: anti- Rabbit biotinylated
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Cells were fixed in ice cold methanol, blocked over night in 1% BSA & 2% serum, Incubated at room temperature for 2hrs with the primary antibody and 1hr with the secondary antibody. The permeabilization step was avoided as the protein of interest is expected on the membrane/cell surface
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投稿 Jun 08 2012