Application
Western blot
Sample
Mouse Cell lysate - whole cell (Bone marrow derived macrophages)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
24 µg
Treatment
crude LPS 600ng/ml for 16 hours and untreated
Specification
Bone marrow derived macrophages
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: ECL Anti-Rabbit IgG, Horseradish Peroxidase linked
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
FEMTO
Exposure
15 minute(s) and 0 second(s)
Additional data
Abcam response
Thank you for your valuable feedback. We have not previously tested this antibody on mouse so it may not be suitable for use in this species. We welcome other abreviews to determine this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Melissa Wallin
Verified customer
投稿 May 12 2014