Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (tumor xenografts from human glioma cells)
Specification
tumor xenografts from human glioma cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TX-100
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% serum in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Mar Lorente
Verified customer
投稿 Jul 28 2009