Application
Western blot
Sample
Human Cell lysate - whole cell (normal human fibroblasts)
Loading amount
60 µg
Specification
normal human fibroblasts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Other product details
Dilution
1/250
Incubation time
24 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: sheep anti-mouse HRP conjugated IgG
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
15 minute(s) and 0 second(s)
Positive control
Human fibroblasts overexpressing a a tagged TRF1 vector
Negative control
not applicable
Additional data
Abcam response
In this case trouble-shooting did not improve this customer's results and a replacement or refund was offered as part of Abcam's Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 08 2006