Anti-TREM2 抗体 (ab95470)
Key features and details
- Goat polyclonal to TREM2
- Suitable for: ICC
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-TREM2 antibody
TREM2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to TREM2 -
由来種
Goat -
アプリケーション
適用あり: ICCmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat -
免疫原
Synthetic peptide corresponding to Mouse TREM2 aa 154-165 (internal sequence).
Sequence:C-QVEHSTSRNQET
Database link: Q99NH8 -
ポジティブ・コントロール
- ICC: NIH3T3-L1 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab95470 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95470の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 10 mg/ml.
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特記事項 |
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ICC
Use a concentration of 10 mg/ml. |
ターゲット情報
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機能
May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. -
組織特異性
Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord. -
関連疾患
Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 83433 Mouse
- Entrez Gene: 301227 Rat
- SwissProt: Q99NH8 Mouse
- Unigene: 261623 Mouse
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別名
- TREM 2 antibody
- TREM-2 antibody
- TREM2 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence analysis of paraformaldehyde fixed mouse 3T3-L1 cells labeling TREM2 with ab95470 at 10 µg/mL (incubation time, 1 hour). Cells were permeabilized with 0.15% Triton. Alexa Fluor 488 secondary antibody was used at 2µg/mL. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10 µg/mL followed by Alexa Fluor 488 secondary antibody 2 µg/mL.
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Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation with ab95470 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab95470 は 7 報の論文で使用されています。
- Sebastian A et al. Single-cell RNA-Seq reveals changes in immune landscape in post-traumatic osteoarthritis. Front Immunol 13:938075 (2022). PubMed: 35967299
- Koronyo-Hamaoui M et al. Peripherally derived angiotensin converting enzyme-enhanced macrophages alleviate Alzheimer-related disease. Brain 143:336-358 (2020). PubMed: 31794021
- Guo Y et al. TREM2 deficiency aggravates a-synuclein-induced neurodegeneration and neuroinflammation in Parkinson's disease models. FASEB J N/A:fj201900992R (2019). PubMed: 31370707
- Ji J et al. The Intra-nuclear SphK2-S1P Axis Facilitates M1-to-M2 Shift of Microglia via Suppressing HDAC1-Mediated KLF4 Deacetylation. Front Immunol 10:1241 (2019). PubMed: 31214192
- Wang ECE et al. A Subset of TREM2+ Dermal Macrophages Secretes Oncostatin M to Maintain Hair Follicle Stem Cell Quiescence and Inhibit Hair Growth. Cell Stem Cell 24:654-669.e6 (2019). PubMed: 30930146
- Jendresen C et al. The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway. J Neuroinflammation 14:59 (2017). PubMed: 28320424
- Raha AA et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). WB, IHC ; Mouse . PubMed: 27662313